![]() Go to Analyze→Gels→Select next lane – Can also use key command “Ctrl+2” – A tiny “2” will appear in the laneĩ. ![]() Make sure your cursor shows as an arrow, grab the rectangle you just made, and drag it to the next lane – DO NOT DRAW A NEW RECTANGLE! You must drag the same rectangle you just made – The point here is to compare the band in each subsequent lane using the exact same size/white space/noise as the originally defined area in Lane 1Ĩ. Go to Analyze→Gels→Select first lane – Can also use key command “Ctrl+1” – A tiny “1” will appear in the laneħ. Select the rectangle tool, and draw a box around the lane, making sure to include some of the empty gel between lanes and white space outside of the bandĦ. Find the lane with the lowest concentration of BSAĥ. – Image→Adjust→Brightness/contrast – I recommend saving the image with an updated name at this point so that you have it to go back toĤ. Does your image look too dark or too light?.– Make sure you save your gel images as the same type of image (either. jpg (in case the tif file can’t be opened-an issue I am experiencing at the other lab). After running and destaining the gel, take a picture and save it as a. ![]() ![]() – For 5GB1, BSA works great as a protein standard, and a range of 0.025 μg/μL to 5.0 μg/μL works well as a range for the standard curve To determine protein concentration you will need to have a standard curve to compare your samples to.Determining the concentration of protein in SDS-PAGE gel bands using ImageJ ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |